The next generation: using new sequencing technologies to analyse gene regulation.

摘要: Next generation sequencing (NGS) has pushed back the limitations of prior technologies to advance genomic knowledge infinitely by allowing cost-effective, rapid become a reality. Genome-wide transcriptional profiling can be achieved using NGS with either Tag-Seq, in which short tags cDNA represent gene, or RNA-Seq, entire transcriptome is sequenced. Furthermore, level and diversity miRNA within different tissues cell types monitored specifically small RNA. The biological mechanisms underlying differential gene regulation also explored coupling chromatin immunoprecipitation (ChIP-Seq). Using this methodology genome-wide binding sites for transcription factors, RNAP II, epigenetic modifiers distribution modified histones assessed. superior, high-resolution data generated adopting technology allows researchers distinguish precise location bound protein correlate observed expression patterns. Additional methods have been established examine other factors influencing such as DNA methylation conformation on scale. Within any research setting, these techniques provide relevant answer numerous questions about regulation. advances made pairing strategic experimental protocols will continue impact community.