Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities.
作者： Takuro Nunoura , Hanako Oida , Junichi Miyazaki , Ai Miyashita , Hiroyuki Imachi
DOI: 10.1111/J.1574-6941.2008.00451.X
关键词: Biology 、 Biochemistry 、 16S ribosomal RNA 、 Primer (molecular biology) 、 Polymerase chain reaction 、 Methanogen 、 Methanobacteria 、 Gene 、 Microbial population biology 、 Methanogenesis
摘要: A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set the methyl coenzyme M reductase subunit gene (mcrA). The developed applied to several microbial communities in which diversity abundance of methanogens or anaerobic methanotrophs (ANMEs) identified by 16S rRNA clone analysis, strong correlations between copy numbers mcrA with those archaeal genes were observed. assay can be assessing and/or ANMEs anoxic environments that could not detected sequence analyses.
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