Scaffold curvature-mediated novel biomineralization process originates a continuous soft tissue-to-bone interface

摘要: Abstract A myriad of shapes are found in biological tissues, often naturally evolved to fulfill a particular function. In the field tissue engineering, substrate geometry influences cell behavior and formation vitro, yet little is known how this translates an vivo scenario. Here we investigate scaffold curvature-induced growth, without additional growth factors or cells, ovine animal model. We show that soft follows curvature-driven The highly organized endogenous matrix, potentially under mechanical strain, leads non-standard form biomineralization, whereby pre-existing organic matrix mineralized collagen remodeling intermediate cartilage ossification phase. Micro- nanoscale characterization microstructure using histology, backscattered electron (BSE) second-harmonic generation (SHG) imaging synchrotron small angle X-ray scattering (SAXS) revealed (i) continuous fibers across soft-hard interface on tip cones, (ii) bone by basic multicellular units (BMUs) regions adjacent native cortical bone. Thus, features tissue-to-bone resembling insertion sites ligaments tendons into were created, did not mimic structural gradients such complex at its mature state. This study provides fundamental knowledge for biomimetic design fields regeneration engineering. Statement significance Geometry vitro. However, influence mean surface curvature Based multiscale characterization, seamless integration newly formed bone, was created cells recapitulate These findings have important implications