Lymphokine mRNA expression by transplantable murine B lymphocytic malignancies. Tumor-derived IL-10 as a possible mechanism for modulating the anti-tumor response.

摘要: In vitro and in vivo expressions of cytokine mRNAs by four transplantable murine B lymphocytic malignancies designated A20, MOPC 315, 2PK-3, RAW 8.1 were determined using sensitive reverse-transcribed (RT)-PCR. Despite significant differences both the stage cell differentiation represented each line method used to induce original tumors, IL-6 IL-10 detected cultured lines. Whereas IL-2, IL-4, IL-5, IL-12 not cells, expression solid tumor tissue was quite different. RT-PCR poly(A)+ RNA isolated from tumors demonstrated presence IL-6, IL-10, TGF-beta 1, TNF-alpha mRNAs. There a noticeable lack IL-2 mRNA any tumors. Using RT-PCR, it clear that malignant lymphocytes expressed TNF-alpha, with limited IL-4 IL-5. To explore mechanisms might contribute these quantitative competitive (QC)-RT-PCR quantify mRNA. 315 most (23.2 pg/micrograms RNA), whereas 7.4, 2.6, 0.6 RNA, respectively. Secretion into culture supernatants or sera ascitic fluid tumor-bearing animals correlated expression. This dysregulated production suggested mechanism may account for possible which limit cell-mediated antitumor responses.