Tunable protein degradation in bacteria
作者： D Ewen Cameron , James J Collins
DOI: 10.1038/NBT.3053
关键词: Bacterial Processes 、 Protein degradation 、 Lactococcus lactis 、 Microbiology 、 Synthetic biology 、 Biology 、 Bacteria 、 Essential gene 、 Computational biology 、 Bacterial genetics 、 Escherichia coli
摘要: Tunable control of protein degradation in bacteria would provide a powerful research tool. Here we use components the Mesoplasma florum transfer-messenger RNA system to create synthetic that provides both independent steady-state level and inducible targeted proteins Escherichia coli. We demonstrate application this circuit development core bacterial processes antibacterial targets, transfer Lactococcus lactis establish its broad functionality bacteria. 238-member library tagged essential E. coli can serve as tool study gene function an applied for antibiotic discovery. Our is modular, does not require disruption host systems be transferred diverse with minimal modification.
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