Isolation of equine bone marrow‐derived mesenchymal stem cells: a comparison between three protocols

摘要: Summary Reason for performing the study: There is a need to assess and standardise equine bone marrow (BM) mesenchymal stem cell (MSC) isolation protocols in order permit valid comparisons between therapeutic trials at different sites. Objective: To compare 3 of BM MSC isolation: adherence plastic culture dish (Classic) 2 gradient density separation (Percoll Ficoll). Materials methods: aspirates were harvested from sternum 6 mares MSCs isolated by all protocols. The viability after isolation, yield, number attained 14 days functional characteristics (self-renewal (CFU) multilineage differentiation capacity) determined protocols. Results: mean ± s.d. yield Percoll protocol was significantly higher (6.8 3.8%) than Classic (1.3 0.7%). numbers recovered per 10 ml sample 24.0 12.1, 14.6 9.5 4.1 2.5 × 106 Percoll, Ficoll protocols, respectively, compared with protocol. Importantly, no significant difference or osteogenic chondrogenic identified At Passage 0, cells retrieved had lower self-renewal capacity when but there 1. differences global frequencies CFUs 0 1. Conclusions clinical relevance: These data suggest that best terms potential lowest only passage 0. Then, equivalent. However, should be considered minimise time.