Cell cycle-specific association of E2F with the p130 E1A-binding protein.

摘要: Association of the E2F transcription factor with pRb and p107 proteins appears to regulate activity and, in turn, affect cell cycle progression. We found, however, that are only minor E2F-associated G0/G1 mouse fibroblasts, we sought identify major partner protein these cells. Because adenovirus E1A oncoprotein seemed able bind G0 partner, enriched for associated both an E2F-binding site DNA column E1A. The species early G1 fibroblasts detected this approach had properties identical pRb- p107-related p130 protein. In serum-stimulated cells, replaced as near G1/S border, concomitant increase levels. p130-E2F complexes resembled p107-E2F their ability cyclin-cdk kinases, they appeared be cyclin E-cdk2 kinase late These observations indicate factors regulated by a succession which associate during defined stages cycle.