Gastric inhibitory polypeptide mechanisms of augmenting insulin secretion
作者： Claude K. Lardinois , Robert B. Richeson , Gale H. Starich , Diane Mazzu , Ernest L. Mazzaferri
DOI: 10.1016/0024-3205(90)90473-5
关键词: Islet 、 Internal medicine 、 Gastrointestinal hormone 、 Intracellular 、 Pancreatic hormone 、 Insulin 、 Gastric inhibitory polypeptide 、 Enzyme inhibitor 、 Biology 、 Phospholipase A2 、 Endocrinology
摘要: The cellular mechanisms whereby gastric inhibitory polypeptide (GIP) augments glucose-dependent insulin secretion remains poorly defined. Since is modulated by membrane associated phospholipase A2 (PLA2) and intracellular lipoxygenase (LPX) cyclooxygenase (CO) we hypothesize that GIP's augmentation of involves these enzyme systems. Neonatal rat pancreatic islet cell cultures were preincubated with 5.6mM glucose for 60 minutes. then stimulated minutes 16mM alone or GIP without the addition PLA2, LPX, CO inhibitors. Insulin significantly increased (P<0.05) when concentration was raised from 5.6 to this further augmented (P<0.05). PLA2 inhibitors (P<0.025) decreased but restored simultaneous GIP. LPX (P<0.01) decrease parsisted despite Simultaneous treatment yielded responses indistinguishable inhibition alone. These studies suggest exerts its influence in part modulating activity. Furthermore, formation products appears be a pivotal step insulinotrophic action
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sci-hub.se 参考文章(35)
S Yamamoto, M Ishii, T Nakadate, T Nakaki, R Kato, Modulation of insulin secretion by lipoxygenase products of arachidonic acid. Relation to lipoxygenase activity of pancreatic islets. Journal of Biological Chemistry. ,vol. 258, pp. 12149- 12152 ,(1983) , 10.1016/S0021-9258(17)44149-4
WILLIAM L. CHICK, ARTHUR A. LIKE, VILMA LAURIS, Pancreatic beta cell culture: preparation of purified monolayers. Endocrinology. ,vol. 96, pp. 637- 643 ,(1975) , 10.1210/ENDO-96-3-637
L. Best, A. Sener, P.C.F. Mathias, W.J. Malaisse, Inhibition by mepacrine and p-bromophenacylbromide of phosphoinositide hydrolysis, glucose oxidation, calcium uptake and insulin release in rat pancreatic islets Biochemical Pharmacology. ,vol. 33, pp. 2657- 2662 ,(1984) , 10.1016/0006-2952(84)90641-5
Stewart A. Metz, Is phospholipase A2 a “glucose sensor” responsible for the phasic pattern of insulin release? Prostaglandins. ,vol. 27, pp. 147- 158 ,(1984) , 10.1016/0090-6980(84)90228-4
S.G. Laychock, Phospholipase A2 activity in pancreatic islets is calcium-dependent and stimulated by glucose Cell Calcium. ,vol. 3, pp. 43- 54 ,(1982) , 10.1016/0143-4160(82)90036-7
John Turk, Bryan A Wolf, Michael L McDaniel, The role of phospholipid-derived mediators including arachidonic acid, its metabolites, and inositol triphosphate and of intracellular Ca2+ in glucose-induced insulin secretion by pancretic islets Progress in Lipid Research. ,vol. 26, pp. 125- 181 ,(1987) , 10.1016/0163-7827(87)90010-5
Stewart A. Metz, Feedback moduiaticn of gilcose-induced insulin secretion by arachidonic acid meiabol: Possible molecular mechanisms and relevance to diabetes mellitus Prostaglandins and Medicine. ,vol. 7, pp. 581- 589 ,(1981) , 10.1016/0161-4630(81)90048-3
R. C. Murphy, W. Fujimoto, S. A. Metz, Effects on Glucose-induced Insulin Secretion of Lipoxygenase-derived Metabolites of Arachidonic Acid Diabetes. ,vol. 33, pp. 119- 124 ,(1984) , 10.2337/DIAB.33.2.119
MICHAEL R. MACADAMS, SUMER BELBEZ PEK, WILLIAM E. M. LANDS, The Effect of Flurbiprofen, a Potent Inhibitor of Prostaglandin Synthesis, on Insulin and Glucagon Release from Isolated Rat Pancreas Endocrinology. ,vol. 114, pp. 1364- 1370 ,(1984) , 10.1210/ENDO-114-4-1364
SAMUEL CATALAND, SAMUEL E. CROCKETT, JOHN C. BROWN, ERNEST L. MAZZAFERRI, Gastric Inhibitory Polypeptide (GIP) Stimulation by Oral Glucose in Man The Journal of Clinical Endocrinology and Metabolism. ,vol. 39, pp. 223- 228 ,(1974) , 10.1210/JCEM-39-2-223