Expresión y función de la proteína quinasa activada por AMP, AMPK, en la célula germinal masculina de cerdo

摘要: espanolEl espermatozoide es una celula germinal altamente especializada que debe experimentar unos procesos funcionales caracteristicos para lograr su funcion primordial: la fecundacion del ovocito. Estos son por un lado dependientes estado energetico, y otro estan regulados mecanismos bioquimicos como fosforilacion de proteinas. La proteina quinasa activada AMP, AMPK, actua molecula sensora carga energetica celular, activacion provoca regulacion metabolismo. Los resultados presente Tesis nos permiten elaborar las siguientes conclusiones: 1- se expresa en el cerdo encuentra enzimaticamente activa condiciones fisiologicas. 2- AMPK localiza principalmente acrosoma pieza intermedia flagelo. 3- ruta senalizacion esta regulada calcio, cAMP, A (PKA), C (PKC) proteinas quinasas ? y/o CAMKK. 4- involucrada movilidad espermatozoide. 5- desempena importante membranas 6- implicada potencial membrana mitocondrial. 7- repuesta a determinados tipos estres celular. En resumen, consideramos los Doctoral proponer esencial regula capacidad masculina adaptarse fluctuantes externas, ocurre durante transito traves tracto reproductor femenino. EnglishSpermatozoon is very specialized germ cell that undergoes characteristic functional processes prior accomplishing its essential function: fertilization of the oocyte. These depend on energy charge this and are also controlled by biochemical mechanisms, such as protein phosphorylation. AMP-activated kinase detects state upon activation regulates metabolism. Results Thesis allow us to establish following conclusions: expressed in boar spermatozoa active under physiological conditions. mainly localized acrosome middle piece flagellum. Intracellular mechanisms leading phosphorylation involved calcium well signalling pathways (PKC), and/or CaMKK. Kinase regulation motility spermatozoa. 5-AMPK plays relevant function membranes. control mitochondrial membrane potential spermatozoa.7- becomes activated response several stresses. In summary, we consider results elaborated allows our proposal acts an male cells ability for adapting changing conditions extracellular medium, it occurs during their transit though female reproductive tract fertilization.