Investigating Metabolic Control of Persister Formation in Biofilms

摘要: Abstract : Bacterial persistence is a phenomenon in which small fraction of bacterial population enters dormancy otherwise growth-promoting conditions to survive future stress. These survivors are responsible for the relapse biofilm infections, and thus greater understanding their formation will lead more effective therapies against biofilm-utilizing pathogens, such as Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus. We have discovered that diauxic carbon shifts stimulate generation persisters planktonic cultures, hypothesized metabolic transitions generate biofilms. In this project, we identifying communities E. P. S. aureus persisters, using genetic biochemical techniques reconstruct underlying signaling pathways. During reporting period specific source coli biofilms persister through ppGpp nucleoid-associated protein dependent pathway. addition, preliminary data suggests nitrogen aeruginosa formation. Further, developed rapid method assay activity. Support from grant has led three publications one submitted manuscript far.