Phosphorylatable short peptide conjugation for facilitating transfection efficacy of CS/DNA complex.

摘要: Previously, we had demonstrated that enhancing intracellular unpacking of exogene from its chitosan carrier by promoting degradation could markedly improve transfection efficiency the CS/DNA complex. In this article addressed a novel strategy phosphorylatable short peptide modification for further facilitating DNA and optimizing A (SP) with amino acid composition "LLLRRRDNEY*FY*VRRLL" containing two potentially tyrosine residues was synthesized. The be phosphorylated constitutively expressed cytoplasmic protein kinase Jak2. SP conjugated to combined GFP/luciferase reporter gene plasmid form SP-CS/DNA vitro phosphorylation releasing assays verified mammalian cell lysate effectively phosphorylate hence promote SP-CS carrier. Thereafter, C2C12 myoblast cells were transfected presented expression GFP luciferase reporters. Further more, multiple lines complexes loading gene. Results revealed that, compared CS, intensively augment level near lipofectamine 2000.