Quantitative threefold allele-specific PCR (QuanTAS-PCR) for highly sensitive JAK2 V617F mutant allele detection

作者: Giada V Zapparoli , Robert N Jorissen , Chelsee A Hewitt , Michelle McBean , David A Westerman

DOI: 10.1186/1471-2407-13-206

关键词: Locked nucleic acidGeneticsFalse positive paradoxMinimal residual diseasePrimer (molecular biology)Variants of PCRAlleleMedicineMutantReal-time polymerase chain reactionMolecular biology

摘要: … , Sanger sequencing, pyrosequencing, amplification refractory mutation system, allele-competitive blocker PCR, and melt curve analysis or high resolution melting (HRM) [7–20]. …

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