High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour.

摘要: Organic light emitting diodes (OLEDs) are in widespread use today’s mobile phones and likely to drive the next generation of large area displays solid-state lighting. Here we show steps towards their utility as a platform technology for biophotonics, by demonstrating devices capable optically controlling behaviour live animals. Using with pin OLED architecture, sufficient illumination intensity (0.3 mW.mm−2) activate channelrhodopsins (ChRs) vivo was reliably achieved at low operating voltages (5 V). In Drosophila melanogaster third instar larvae expressing ChR2(H134R) motor neurons, found that pulsed from blue green OLEDs triggered robust reversible contractions This response temporally coupled timing illumination. With illumination, initial rate overall size behavioural strongest. Green roughly 70% observed OLEDs. Orange did not produce larvae, agreement spectral ChR2(H134R). The device configuration presented here could be modified accommodate other small model organisms, cell cultures or tissue slices ability provide patterned tuning can further broaden optogenetics experiments.