Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.
作者： Susanne Pauline Roth , Walter Brehm , Claudia Groß , Patrick Scheibe , Susanna Schubert
DOI: 10.3390/IJMS20215474
关键词: Cell biology 、 Tenascin C 、 Tissue engineering 、 Transforming growth factor, beta 3 、 Decellularization 、 Chemistry 、 Tendon 、 Scaffold 、 Surface coating 、 Decorin
摘要: Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed decellularized equine superficial digital flexor scaffolds, investigate the bioactivity of scaffold-associated an vitro model. could effectively be loaded onto scaffolds so that at least 88% applied were not detected rinsing fluid TGFβ3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) then seeded on with 300 ng assess its bioactivity. Both dissolved cell culture medium, latter serving as control group, promoted elongation shapes scaffold contraction (p < 0.05). Furthermore, affected MSC musculoskeletal gene expression a similar manner, upregulation tenascin c downregulation other matrix molecules, most markedly decorin These results demonstrate is preserved, thus application via absorption feasible approach.
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