Erythropoietin Promotes Survival of Retinal Ganglion Cells in DBA/2J Glaucoma Mice

摘要: Purpose Retinal ganglion cell (RGC) loss occurs in response to increased intraocular pressure (IOP) and/or retinal ischemia glaucoma and leads impairment of vision. This study was undertaken test the efficacy erythropoietin (EPO) providing neuroprotection RGCs vivo. Methods The neuroprotective effects EPO were studied DBA/2J mouse model glaucoma. Mice intraperitoneally injected with control substances or various doses EPO, starting at age 6 months continuing for an additional 2, 4, months. labeled retrogradely by a gold tracer. IOP measured microelectric-mechanical system, receptor (EPOR) expression detected immunohistochemistry. Axonal death optic nerve quantified para-phenylenediamine staining, complete blood count system used measure number erythrocytes. Results In mice, average viable significantly decreased from 4 10 months, inverse correlation between dead axons RGCs. Treatment 3000, 6000, 12,000 U/kg body weight per week all prevented significant RGC loss, compared untreated animals. similar those memantine, known agent. IOP, contrast, unchanged both memantine. Finally, EPOR expressed layer C57BL/6J mice. Conclusions promoted survival glaucomatous mice without affecting IOP. These results suggest that may be potential therapeutic neuroprotectant