Generation of thalamic neurons from mouse embryonic stem cells
作者： Atsushi Shiraishi , Keiko Muguruma , Yoshiki Sasai
DOI: 10.1242/DEV.144071
关键词: Thalamus 、 Neuroscience 、 Biology 、 Neurite 、 Transplantation 、 Neural tube 、 Forebrain 、 Embryoid body 、 Cell aggregation 、 Neocortex 、 Anatomy
摘要: The thalamus is a diencephalic structure that plays crucial roles in relaying and modulating sensory motor information to the neocortex. develops dorsal part of neural tube at level caudal forebrain. However, molecular mechanisms are essential for thalamic differentiation still unknown. Here, we have succeeded generating neurons from mouse embryonic stem cells (mESCs) by modifying default method induces most-anterior type self-organizing culture. A low concentration caudalizing factor insulin MAPK/ERK kinase inhibitor enhanced expression forebrain markers Otx2 Pax6. BMP7 promoted an increase precursors such as Tcf7l2+/Gbx2+ Tcf7l2+/Olig3+ cells. mESC began express glutamate transporter vGlut2 axon-specific marker VGF, similar mature projection neurons. extended their axons cortical layers both organotypic culture subcortical transplantation. Thus, identified minimum elements sufficient vitro generation These findings expand our knowledge development.
biologists.com
biologists.org参考文章(52)
Yasushi Nakagawa, Dennis D. M. O'Leary, Combinatorial Expression Patterns of LIM-Homeodomain and Other Regulatory Genes Parcellate Developing Thalamus The Journal of Neuroscience. ,vol. 21, pp. 2711- 2725 ,(2001) , 10.1523/JNEUROSCI.21-08-02711.2001
Keiko Muguruma, Yoshiki Sasai, In vitro recapitulation of neural development using embryonic stem cells: From neurogenesis to histogenesis Development, Growth & Differentiation. ,vol. 54, pp. 349- 357 ,(2012) , 10.1111/J.1440-169X.2012.01329.X
E.J. Robertson, M.J. Solloway, Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development. ,vol. 126, pp. 1753- 1768 ,(1999) , 10.1242/DEV.126.8.1753
E. Robertshaw, K. Matsumoto, A. Lumsden, C. Kiecker, Irx3 and Pax6 establish differential competence for Shh-mediated induction of GABAergic and glutamatergic neurons of the thalamus Proceedings of the National Academy of Sciences of the United States of America. ,vol. 110, pp. 201304311- ,(2013) , 10.1073/PNAS.1304311110
Kiichi Watanabe, Daisuke Kamiya, Ayaka Nishiyama, Tomoko Katayama, Satoshi Nozaki, Hiroshi Kawasaki, Yasuyoshi Watanabe, Kenji Mizuseki, Yoshiki Sasai, Directed differentiation of telencephalic precursors from embryonic stem cells Nature Neuroscience. ,vol. 8, pp. 288- 296 ,(2005) , 10.1038/NN1402
Claudia Vieira, Ana-Lila Garda, Kenji Shimamura, Salvador Martinez, Thalamic development induced by Shh in the chick embryo. Developmental Biology. ,vol. 284, pp. 351- 363 ,(2005) , 10.1016/J.YDBIO.2005.05.031
K. K. Bluske, T. Y. Vue, Y. Kawakami, M. M. Taketo, K. Yoshikawa, J. E. Johnson, Y. Nakagawa, β-Catenin signaling specifies progenitor cell identity in parallel with Shh signaling in the developing mammalian thalamus Development. ,vol. 139, pp. 2692- 2702 ,(2012) , 10.1242/DEV.072314
Wolfgang Wurst, Laure Bally-Cuif, Neural plate patterning: Upstream and downstream of the isthmic organizer Nature Reviews Neuroscience. ,vol. 2, pp. 99- 108 ,(2001) , 10.1038/35053516
Makoto Ikeya, Masako Kawada, Yoko Nakazawa, Makoto Sakuragi, Noriaki Sasai, Morio Ueno, Hiroshi Kiyonari, Kazuki Nakao, Yoshiki Sasai, Gene disruption/knock-in analysis of mONT3: Vector construction by employing both in vivo and in vitro recombinations The International Journal of Developmental Biology. ,vol. 49, pp. 807- 823 ,(2005) , 10.1387/IJDB.051975MI
Nobuhiko Yamamoto, Katsuya Yamada, Tohru Kurotani, Keisuke Toyama, Laminar specificity of extrinsic cortical connections studied in coculture preparations Neuron. ,vol. 9, pp. 217- 228 ,(1992) , 10.1016/0896-6273(92)90161-6