Q-switched 1064-nm dymium-doped yttrium aluminum garnet laser irradiation induces skin collagen synthesis by stimulating MAPKs pathway.

摘要: The 1064-nm Q-switched neodymium-doped yttrium aluminum garnet (Nd:YAG) laser is widely used in clinical practice. However, the effects of Nd:YAG on skin collagen generation have not been fully elucidated. objectives present study were to investigate whether can be for non-ablative rejuvenation and explore possible mechanism underlying effects. Six-week-old SKH-1 hairless mice irradiated by at fluences 0, 0.5, 1, 1.5, 2 J/cm2, respectively. contents hydroxyproline hydration detected after irradiation. Moreover, hematoxylin-eosin (HE) staining was preformed evaluate dermal thickness. Immunofluorescence detect expressions MMP-2 TIMP-1 Furthermore, qRT-PCR performed determine TGF-β1 Smad3. In addition, ERK1/2, p-ERK1/2, p38, p-p38, JNK, ERK5, evaluated Western blotting. results indicated that levels hydroxyproline, hydration, markedly increased; both thickness enhanced low dose treatment. expression significantly increased, whereas remarkably decreased Meanwhile, TGF-β1, Smad3, p-P38, JNK productions group compared with ones non-irradiated. Nevertheless, no significant changes observed ERK5 summary, our demonstrated induce generation, least part, through activating TGF-β1/Smad3/MAPK signaling pathway.