CD40 Ligation Enhances IL-15 Production by Tubular

摘要: Interleukin-15 (IL-15) is a potent T-cell growth fac- tor and activator. Acute rejection of kidney allografts strongly correlated with elevated IL-15 mRNA in the graft. A role process also suggested for interaction between CD40 ligand (CD154) expressed on membranes activated T cells its receptor (CD40). The effect ligation production human tubular epithelial (TEC) was investigated. TEC were co-cultured cell line genetically engineered to express CD154. CD154-expressing (CD40L cells) bind TEC. Addition CD40L culture resulted levels. This enhanced not observed control cells, when anti- CD154 antibody added, direct contact CD40L-cells prevented use Trans- well system. activation twofold increase transcripts as measured by reverse transcription- PCR concordant elevation protein specific enzyme-linked immunosorbent assay. intensity linearly dependent number, reaching plateau effector/target-ratio 1:1. levels similar that produced stimulation interferon-g (IFN-g). Combina- tion IFN-g an addi- tive effect. To conclude, may enhance expression two ways: secreting using Each signal alone induces magnitudes, both signals are additive. Because major activator, this contrib- ute graft rejection.