The R elative Retrotransposition Effic iencies of Human and Mouse Short Interspersed DNA Elements (SINEs) in a HeLa Cell Culture Assay

摘要: Short interspersed DNA elements (SINEs) and long (LINEs) represent two families of transposable (TEs) within mammalian genomes. About 45% the human genome consists TE derived sequences. SINEs do not encode protein, so they cannot be autonomously propagated require additional cellular machinery, most likely LINE proteins (ORF1p ORF2p), for their mobilization, which has been supported by a cell culture-based retrotransposition assay. are ancestrally from either 7SL RNA gene or various tRNA genes. Sequence data support co-evolution some tRNA-derived with LINEs. This study involved testing whether tRNAderived could mobilize in absence ORF1p, as well assessing coevolution both LINEs using A series SINE constructs containing Alu, B2, B1 were created analyzed. No evidence to was found; however, it discovered that ORF1p mobilize. Interestingly, RNA-derived more effectively ORF1p.