Assessment of endogenous reference gene suitability for serum exosomal microRNA expression analysis in liver carcinoma resection studies.
作者： YI LI , GUI-MING XIANG , LIN-LIN LIU , CHANG LIU , FEI LIU
关键词: Hepatocellular carcinoma 、 Molecular medicine 、 Gene 、 Reference genes 、 Polymerase chain reaction 、 Candidate gene 、 Oncogene 、 Biology 、 Bioinformatics 、 Cancer research 、 microRNA
摘要: Abstract Serum exosomal microRNAs (miRNAs) have received considerable attention as potential biomarkers for tumor diagnosis. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) is commonly used to detect miRNA expression levels in various types of cancer. One prerequisite valid RT‑qPCR data the correct normalization miRNAs stably expressed endogenous reference genes (RGs). The study liver carcinoma resection requires use reliable RGs order assess serum target miRNAs. However, assessment RG suitability optimum analysis has yet be investigated. present investigated stability 10 candidate RGs. included eight (miR‑16, miR‑103, miR‑191, let‑7a, miR‑26a, miR‑221, miR‑181a, and miR‑451) two small RNAs (5S U6). values were calculated using following algorithms: geNorm, NormFinder, BestKeeper, comparative ΔCt method. overall ranking obtained from these analyses revealed that miR‑26a appropriate internal patients with hepatocellular carcinoma. In addition, miR‑221 let‑7a combined, recommended by or increased accuracy interpretation hepatopathy studies.
sci-hub.se 参考文章(38)
A Michael, SD Bajracharya, PST Yuen, H Zhou, RA Star, GG Illei, I Alevizos, Exosomes from human saliva as a source of microRNA biomarkers. Oral Diseases. ,vol. 16, pp. 34- 38 ,(2010) , 10.1111/J.1601-0825.2009.01604.X
Stephen A Bustin, Tania Nolan, Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. Journal of biomolecular techniques. ,vol. 15, pp. 155- 166 ,(2004)
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Clotilde Théry, Sebastian Amigorena, Graça Raposo, Aled Clayton, Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids Current protocols in pharmacology. ,vol. 30, ,(2006) , 10.1002/0471143030.CB0322S30
Hai-Tao Zhu, Qiong-Zhu Dong, Guan Wang, Hai-Jun Zhou, Ning Ren, Hu-Liang Jia, Qing-Hai Ye, Lun-Xiu Qin, Identification of Suitable Reference Genes for qRT-PCR Analysis of Circulating microRNAs in Hepatitis B Virus-Infected Patients Molecular Biotechnology. ,vol. 50, pp. 49- 56 ,(2012) , 10.1007/S12033-011-9414-6
Ilaria Giusti, Sandra D’Ascenzo, Vincenza Dolo, Microvesicles as Potential Ovarian Cancer Biomarkers BioMed Research International. ,vol. 2013, pp. 703048- 703048 ,(2013) , 10.1155/2013/703048
Suresh Mathivanan, Hong Ji, Richard J. Simpson, Exosomes: extracellular organelles important in intercellular communication. Journal of Proteomics. ,vol. 73, pp. 1907- 1920 ,(2010) , 10.1016/J.JPROT.2010.06.006
Guilherme Rabinowits, Cicek Gerçel-Taylor, Jamie M. Day, Douglas D. Taylor, Goetz H. Kloecker, Exosomal microRNA: A Diagnostic Marker for Lung Cancer Clinical Lung Cancer. ,vol. 10, pp. 42- 46 ,(2008) , 10.3816/CLC.2009.N.006
Jessica Wahlgren, Tanya De L. Karlson, Mikael Brisslert, Forugh Vaziri Sani, Esbjörn Telemo, Per Sunnerhagen, Hadi Valadi, Plasma exosomes can deliver exogenous short interfering RNA to monocytes and lymphocytes Nucleic Acids Research. ,vol. 40, ,(2012) , 10.1093/NAR/GKS463
Zhao-You Tang, Sheng-Long Ye, Yin-Kun Liu, Lun-Xiu Qin, Hui-Chuan Sun, Qin-Hai Ye, Lu Wang, Jian Zhou, Shuang-Jian Qiu, Yan Li, Xue-Ning Ji, Hu Liu, Jing-Ling Xia, Zhi-Quan Wu, Jia Fan, Zeng-Chen Ma, Xin-Da Zhou, Zhi-Ying Lin, Kang-Da Liu, A decade's studies on metastasis of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology. ,vol. 130, pp. 187- 196 ,(2004) , 10.1007/S00432-003-0511-1