Effects of Particle Porosity on the Separation of Larger Molecules

摘要: The pore structure of a chromatographic stationary phase accounts for the vast majority surface area responsible separation. diameter influences overall and with it capacity column but also limits size analyte can or should be used for. In adsorption chromatography limiting effect is further enhanced by adsorbed molecules partially blocking structure. analysis large diffusion becomes crucial parameter in efficiency column. A number models deal hindered mass transfer porous systems. Effects particle porosity on separation larger are discussed examples small, medium analytes various phases given. Pore shown to an important when analyzing molecules. By selecting right task trade-off optimized achieve best possible For tryptic digests 200 superior 300 due its two-fold increase while 1000 required proteins.