Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.
作者： Maxwell W. Brown , Yoori Kim , Gregory M. Williams , John D. Huck , Jennifer A. Surtees
DOI: 10.1038/NCOMMS10607
关键词: Genetics 、 Nucleotide excision repair 、 Eukaryotic DNA replication 、 Replication protein A 、 Biology 、 DNA clamp 、 DNA mismatch repair 、 HMG-box 、 DNA repair 、 Mismatch repair complex 、 Cell biology
摘要: DNA-binding proteins search for specific targets via facilitated diffusion along a crowded genome. However, little is known about how DNA modulates and target recognition. Here we use curtains single-molecule fluorescence imaging to investigate Msh2-Msh3, eukaryotic mismatch repair complex, navigates on DNA. Msh2-Msh3 hops over nucleosomes other protein roadblocks, but maintains sufficient contact with recognize single lesion. In contrast, Msh2-Msh6 slides without hopping largely blocked by roadblocks. Remarkably, the Msh3-specific mispair-binding domain (MBD) licences chimeric Msh2-Msh6(3MBD) bypass nucleosomes. Our studies contrast navigate genome suggest locates lesions outside of replication-coupled repair. These results also provide insights into factors in context chromatin.
nature.com
harvard.edu
sci-hub.se 参考文章(71)
Ignacio F. Gallardo, Praveenkumar Pasupathy, Maxwell Brown, Carol M. Manhart, Dean P. Neikirk, Eric Alani, Ilya J. Finkelstein, High-Throughput Universal DNA Curtain Arrays for Single-Molecule Fluorescence Imaging. Langmuir. ,vol. 31, pp. 10310- 10317 ,(2015) , 10.1021/ACS.LANGMUIR.5B02416
D J Wright, K King, P Modrich, The negative charge of Glu-111 is required to activate the cleavage center of EcoRI endonuclease. Journal of Biological Chemistry. ,vol. 264, pp. 11816- 11821 ,(1989) , 10.1016/S0021-9258(18)80138-7
J.M. Sogo, H. Stahl, Th. Koller, R. Knippers, Structure of replicating simian virus 40 minichromosomes: The replication fork, core histone segregation and terminal structures☆ Journal of Molecular Biology. ,vol. 189, pp. 189- 204 ,(1986) , 10.1016/0022-2836(86)90390-6
Stanley D. Chandradoss, Nicole T. Schirle, Malwina Szczepaniak, Ian J. MacRae, Chirlmin Joo, A Dynamic Search Process Underlies MicroRNA Targeting Cell. ,vol. 162, pp. 96- 107 ,(2015) , 10.1016/J.CELL.2015.06.032
Timothy Snowden, Samir Acharya, Charles Butz, Mark Berardini, Richard Fishel, hMSH4-hMSH5 Recognizes Holliday Junctions and Forms a Meiosis-Specific Sliding Clamp that Embraces Homologous Chromosomes Molecular Cell. ,vol. 15, pp. 437- 451 ,(2004) , 10.1016/J.MOLCEL.2004.06.040
Monika Sharma, Alexander V. Predeus, Shayantani Mukherjee, Michael Feig, DNA bending propensity in the presence of base mismatches: implications for DNA repair. Journal of Physical Chemistry B. ,vol. 117, pp. 6194- 6205 ,(2013) , 10.1021/JP403127A
W. H. Lang, J. E. Coats, J. Majka, G. L. Hura, Y. Lin, I. Rasnik, C. T. McMurray, Conformational trapping of Mismatch Recognition Complex MSH2/MSH3 on repair-resistant DNA loops Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 17247- 17248 ,(2011) , 10.1073/PNAS.1105461108
Jong-Bong Lee, Won-Ki Cho, Jonghyun Park, Yongmoon Jeon, Daehyung Kim, Seung Hwan Lee, Richard Fishel, Single-molecule views of MutS on mismatched DNA DNA Repair. ,vol. 20, pp. 82- 93 ,(2014) , 10.1016/J.DNAREP.2014.02.014
Shikha Gupta, Martin Gellert, Wei Yang, Mechanism of mismatch recognition revealed by human MutSβ bound to unpaired DNA loops. Nature Structural & Molecular Biology. ,vol. 19, pp. 72- 78 ,(2012) , 10.1038/NSMB.2175
Murray S. Junop, Galina Obmolova, Kelly Rausch, Peggy Hsieh, Wei Yang, Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair. Molecular Cell. ,vol. 7, pp. 1- 12 ,(2001) , 10.1016/S1097-2765(01)00149-6