Knock out CD44 in reprogrammed liver cancer cell C3A increases CSCs stemness and promotes differentiation
作者： Shuo Han , Jinhai Guo , Yinan Liu , Zhi Zhang , Qihua He
关键词: SOX2 、 Homeobox protein NANOG 、 KLF4 、 Stem cell 、 Pathology 、 Cellular differentiation 、 Stem cell marker 、 CD44 、 Cancer stem cell 、 Cancer research 、 Medicine 、 Oncology
摘要: CD44 is a widely known cancer stem cells marker in various cancers and validated to function tumor growth, survival metastasis. In this study, we first established C3A-derived liver by OSKM method [OCT4, SOX2, KLF4, c-MYC], termed C3A-induced (C3A-iCSCs) which acquired self-renewal stemness abilities. Then found was positive C3A-iCSCs mainly located cell nuclear. Chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) results showed nuclear combined promoter regions of c-MYC SOX2. These suggested that participated transcriptional regulation. To explore overall influence cells, knocked out using CRISPR/Cas9 technology. Our dramatic increase the expression markers OCT4, SOX2 NANOG CD44- compared with CD44+ C3A-iCSCs. Tumor derived from also displayed well-differentiated C3A-iCSCs, exhibited lower malignant degree. data indicated responsible for poorly differentiated highly maintenance low state.
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