Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostrea gigas

作者: Anne-Leila Meistertzheim , Morgane Lejart , Nelly Le Goïc , Marie-Thérèse Thébault

DOI: 10.1016/J.CBPA.2008.10.004

关键词: Aquatic animalCrassostreaHsp70ZoologyGonadBiologyEcologyOysterGametogenesisPacific oysterGillBiochemistryPhysiologyMolecular biology

摘要: Pacific oysters, Crassostrea gigas, living at a range of tidal heights, routinely encounter large fluctuations in temperature. We demonstrate that levels heat shock proteins (HSP) and other stress (metallothioneins, MTs) quantified by ELISA, remained similar gills, mantle digestive gland between oysters inhabiting low high heights. In contrast, endogenous HSPs MTs gonad changed significantly during gametogenesis. female gonads, the constitutive form HSP70 increased from immature (or resting) to mature stages (about more than 3-fold) decreased after spawning. male same expression patterns were observed, whereas once fully mature. Females presented higher concentration males spawning period. No significant difference was found among sampled hypothesize level eggs may increase survival oyster progeny.

参考文章(53)
Pierre Lubey, Recherches sur le cycle sexuel et l'émission des gamètes chez les mytilides et les pectinides (Mollusques bivalves) Revue des Travaux de l'Institut des Pêches Maritimes. ,vol. 23, pp. 387- 548 ,(1959)
D. A. Roberts, G. E. Hofmann, G. N. Somero, Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects. The Biological Bulletin. ,vol. 192, pp. 309- 320 ,(1997) , 10.2307/1542724
Anthony L. Fink, Chaperone-Mediated Protein Folding Physiological Reviews. ,vol. 79, pp. 425- 449 ,(1999) , 10.1152/PHYSREV.1999.79.2.425
Gautier Damiens, Catherine Mouneyrac, Françoise Quiniou, Edouard His, Mauricette Gnassia-Barelli, Michèle Roméo, Metal bioaccumulation and metallothionein concentrations in larvae of Crassostrea gigas Environmental Pollution. ,vol. 140, pp. 492- 499 ,(2006) , 10.1016/J.ENVPOL.2005.08.006
Isabelle Boutet, Arnaud Tanguy, Sabrina Rousseau, Michel Auffret, Dario Moraga, Molecular identification and expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70) genes in the Pacific oyster Crassostrea gigas Cell Stress & Chaperones. ,vol. 8, pp. 76- 85 ,(2003) , 10.1379/1466-1268(2003)8<76:MIAEOH>2.0.CO;2
Michael Martin, Kenneth E. Osborn, Patricia Billig, Neil Glickstein, Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae Marine Pollution Bulletin. ,vol. 12, pp. 305- 308 ,(1981) , 10.1016/0025-326X(81)90081-3
Fabiola Lango-Reynoso, Jorge Chávez-Villalba, Jean Claude Cochard, Marcel Le Pennec, Oocyte size, a means to evaluate the gametogenic development of the Pacific oyster, Crassostrea gigas (Thunberg) Aquaculture. ,vol. 190, pp. 183- 199 ,(2000) , 10.1016/S0044-8486(00)00392-6
A. Hamdoun, D. Epel, Embryo stability and vulnerability in an always changing world Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 1745- 1750 ,(2007) , 10.1073/PNAS.0610108104