Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components.

摘要: Coxiella burnetii, phase I and II, cells cultivated in the yolk sac of chicken embryos were separated from host cell components by two cycles isopycnic Renografin gradient centrifugation. Initial steps purification viable C. burnetii involved differential centrifugation sedimentation through an aqueous solution 30% sucrose 7.6% Renografin. After first, but not second, cycle centrifugation, passed microfilter glass filters which facilitated removal components. The integrity morphologically different variants was maintained during procedures suspending highly purified phosphate-buffered saline-sucrose solutions. phases obtained these methods appeared to be free serological while retaining morphological infectivity for sacs experimental animals. Average yields 2.83, 1.5, 0.84 mg (dry weight) per Ohio strain (phase I), 9 Mile II), respectively. Recovery averaged about 70%, whereas recovery phage II approximately 40%. temporal sequence antibody response demonstrated infected vaccinated Also, no mice guinea pigs antigens detectable after injections vaccine or cells. Importantly, this is first report separation Images