VEGF therapeutic gene delivery using dendrimer type bio-reducible polymer into human mesenchymal stem cells (hMSCs)
作者： Hyojung Kim , Kihoon Nam , Joung-Pyo Nam , Hyun Soo Kim , Yong Man Kim
DOI: 10.1016/J.JCONREL.2015.09.018
关键词: Mesenchymal stem cell 、 Cytotoxicity 、 Genetic enhancement 、 Molecular biology 、 Gene delivery 、 Transfection 、 Dendrimer 、 Stem cell 、 Biology 、 Cell therapy 、 Cell biology
摘要: The therapeutic potential of mesenchymal stem cells (MSCs) has garnered great attention in the expansive diversity biomedical research. Despite this broad interest cells, limited incorporation and poor viability are major disadvantages for accomplishing success field hMSC-based cell therapy, an optimal approach therapy using non-viral vectors not been established. Hence, we examined possibility performing gene biodegradable polymeric vector Arginine-grafted poly (cystaminebisacrylamide-diaminohexane) (ABP)-conjugated (amidoamine) (PAMAM) dendrimer (PAM-ABP) hMSCs. PAM-ABP formed compact nanosized polyplexes showed low cytotoxicity compared to bPEI 25k Lipofectamine® 2000 Although cellular uptake was similar, transfection efficiency VEGF expression gWiz-Luc pβ-VEGF were higher than those control groups. hMSCs transfected, their characteristics retained. Our results suggest that ability deliver a
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