The cytochrome c peroxidase activity of cytochrome oxidase.
作者： Y Orii
DOI: 10.1016/S0021-9258(18)34056-0
关键词: Cyanide 、 Peroxidase 、 Chemistry 、 Cytochrome c oxidase 、 Carbon monoxide 、 Medicinal chemistry 、 Hydrogen peroxide 、 Inorganic chemistry 、 Oxidase test 、 Cytochrome-c peroxidase activity 、 Cytochrome c peroxidase
摘要: I have found that mammalian cytochrome oxidase catalyzes the peroxidatic oxidation of ferrocytochrome c under strictly anaerobic conditions. An apparent Km value for was 2 microM, and a second order rate constant, estimated as an extrapolated value, 1.4 X 10(6) M-1 s-1 at pH 7.4 25 degrees C. These values were quite similar to corresponding 6.4 microM 1.9 determined intrinsic activity. The showed hyperbolic dependence on concentration hydrogen peroxide, ws 0.18 mM. Cyanide azide 0.1 mM inhibited peroxidase activity by 100 98%, respectively, whereas, carbon monoxide 750 mm Hg, 10% still remained. Under air, acted simultaneously peroxidase.
sci-hub.st 参考文章(9)
B. Errede, M.D. Kamen, Youssef Hatefi, [8] Preparation and properties of complex IV (Ferrocytochrome c: Oxygen oxidoreductase EC 1.9.3.1) Methods in Enzymology. ,vol. 53, pp. 40- 47 ,(1978) , 10.1016/S0076-6879(78)53011-5
Takashi Yonetani, Gisela S. Ray, Studies on Cytochrome c Peroxidase Journal of Biological Chemistry. ,vol. 241, pp. 700- 706 ,(1966) , 10.1016/S0021-9258(18)96895-X
Takashi Yonetani, Tomoko Ohnishi, Cytochrome c Peroxidase, a Mitochondrial Enzyme of Yeast Journal of Biological Chemistry. ,vol. 241, pp. 2983- 2984 ,(1966) , 10.1016/S0021-9258(18)96562-2
BUNJI HAGIHARA, ICHIRO MORIKAWA, ICHIRO SEKUZU, TAKEKAZU HORIO, KAZUO OKUNUKI, Crystalline Cytochrome C: Preparation of Crystalline Cytochrome c from Beef and Pig Heart Nature. ,vol. 178, pp. 630- 631 ,(1956) , 10.1038/178630A0
YUTAKA ORII, KAZUO OKUNUKI, STUDIES ON CYTOCHROME A. X. EFFECT OF HYDROGEN PEROXIDE ON ABSORPTION SPECTRA OF CYTOCHROME A. Journal of Biochemistry. ,vol. 54, pp. 207- 213 ,(1963) , 10.1093/OXFORDJOURNALS.JBCHEM.A127773
Yutaka ORII, Masao MANABE, Machiko YONEDA, Molecular architecture of cytochrome oxidase and its transition on treatment with alkali or sodium dodecyl sulfate. Journal of Biochemistry. ,vol. 81, pp. 505- 517 ,(1977) , 10.1093/OXFORDJOURNALS.JBCHEM.A131484
W.W. Wainio, Janet Greenlees, Complexes of cytochrome c oxidase with cyanide and carbon monoxide Archives of Biochemistry and Biophysics. ,vol. 90, pp. 18- 21 ,(1960) , 10.1016/0003-9861(60)90605-6
A. O. Allen, C. J. Hochanadel, J. A. Ghormley, T. W. Davis, Decomposition of Water and Aqueous Solutions under Mixed Fast Neutron and γ-Radiation The Journal of Physical Chemistry. ,vol. 56, pp. 575- 586 ,(1952) , 10.1021/J150497A007
M.V. Gilmour, R. Lemberg, Cytochrome oxidase and its derivatives. VIII. Formation and properties of the ‘oxygenated’ from of cytochrome oxidase and its reconversion to ferrous and ferric oxidase Biochimica et Biophysica Acta. ,vol. 143, pp. 500- 517 ,(1967) , 10.1016/0005-2728(67)90055-2