Clinical and pathologic methods to assess the long‐term safety of nasal corticosteroids

摘要: Background: The main objective of this long-term prospective local safety study was to evaluate endoscopic and histologic changes in nasal epithelium after 6-month treatment with triamcinolone acetonide (TAA). We describe here a method measure quantitatively thickness. Results were compared those seen the use cetirizine (an antihistamine) another oral intranasal corticosteroid, beclomethasone dipropionate (BDP). Methods: Patients examined by an ENT specialist who first performed evaluation cavities, assessing any morphologic abnormalities aspect mucosa. Biopsies taken from inferior turbinate before 24 weeks treatment. immediately fixed cold acetone (−20°C) embedded glycolmethacrylate; sections 2 µm cut on ultramicrotome. Morphometric evaluations done blinded fashion computerized image analysis epithelial area over minimum length 50 µm. The thickness ascertained ratio length. Results: For all three groups, decreased slightly pretreatment end treatment. No statistically significant differences between groups found thickness. Macroscopically, tissues treated normal. Conclusions: These results clearly indicate that TAA has no atrophic effect