Repurposing the scorpion venom peptide VmCT1 into an active peptide against Gram-negative ESKAPE pathogens.
作者： Cibele Nicolaski Pedron , Iris Araújo , Pedro Ismael da Silva Junior , Fernanda Dias da Silva , Marcelo Der Torossian Torres
DOI: 10.1016/J.BIOORG.2019.103038
关键词: Biochemistry 、 Proline 、 Glycine 、 Antimicrobial 、 Proteases 、 Chemistry 、 Venom 、 Peptide 、 Bacteria 、 Structure–activity relationship
摘要: Abstract VmCT1 is a cationic antimicrobial peptide (AMP) from the venom of scorpion Vaejovis mexicanus. and analogs were designed with single substitutions for verifying influence changes in physicochemical features described as important AMPs hemolytic activities, well their effect on resistance against proteases action. The increase net positive charge by introduction an arginine residue positions hydrophilic face helical structure affected directly activity. Arg-substituted presented activity Gram-negative bacteria ESKAPE list pathogens that not observed VmCT1. Additionally, peptides higher increased values ranging 0.39 to 12.5 μmol L−1 Gram-positive fungi. phenylalanine substitution glycine (position 1), valine proline 8) led lower (at concentrations 50 100 μmol L−1, respectively). These results revealed it possible modulate biological activities derivatives designing substituted-analogs prospective therapeutics
sci-hub.se 参考文章(54)
Tomas Rozek, Kate L. Wegener, John H. Bowie, Ian N. Olver, John A. Carver, John C. Wallace, Michael J. Tyler, The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2. FEBS Journal. ,vol. 267, pp. 5330- 5341 ,(2000) , 10.1046/J.1432-1327.2000.01536.X
Santos Ramírez-Carreto, Verónica Quintero-Hernández, Juana María Jiménez-Vargas, Gerardo Corzo, Lourival D. Possani, Baltazar Becerril, Ernesto Ortiz, Gene cloning and functional characterization of four novel antimicrobial-like peptides from scorpions of the family Vaejovidae. Peptides. ,vol. 34, pp. 290- 295 ,(2012) , 10.1016/J.PEPTIDES.2012.02.002
Luyang Cao, Chao Dai, Zhongjie Li, Zheng Fan, Yu Song, Yingliang Wu, Zhijian Cao, Wenxin Li, Antibacterial Activity and Mechanism of a Scorpion Venom Peptide Derivative In Vitro and In Vivo PLoS ONE. ,vol. 7, pp. e40135- ,(2012) , 10.1371/JOURNAL.PONE.0040135
Ran Yan, Zhenhuan Zhao, Yawen He, Lin Wu, Dawei Cai, Wei Hong, Yingliang Wu, Zhijian Cao, Congyi Zheng, Wenxin Li, A new natural α-helical peptide from the venom of the scorpion Heterometrus petersii kills HCV. Peptides. ,vol. 32, pp. 11- 19 ,(2011) , 10.1016/J.PEPTIDES.2010.10.008
Tamar Unger, Ziv Oren, Yechiel Shai, The Effect of Cyclization of Magainin 2 and Melittin Analogues on Structure, Function, and Model Membrane Interactions: Implication to Their Mode of Action Biochemistry. ,vol. 40, pp. 6388- 6397 ,(2001) , 10.1021/BI0026066
Richard M. Epand, Raquel F. Epand, Domains in bacterial membranes and the action of antimicrobial agents Molecular BioSystems. ,vol. 5, pp. 580- 587 ,(2009) , 10.1039/B900278M
Katsuhiro Konno, Miki Hisada, Renato Fontana, Carla C.B. Lorenzi, Hideo Naoki, Yasuhiro Itagaki, Akiko Miwa, Nobufumi Kawai, Yoshihiro Nakata, Tadashi Yasuhara, João Ruggiero Neto, Walter F. de Azevedo, Mario S. Palma, Terumi Nakajima, Anoplin, a novel antimicrobial peptide from the venom of the solitary wasp Anoplius samariensis Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. ,vol. 1550, pp. 70- 80 ,(2001) , 10.1016/S0167-4838(01)00271-0
Sung-Hee Lee, Seo-Jin Kim, Yoo-Sup Lee, Min-Dong Song, Ick-Hee Kim, Hyung-Sik Won, De novo generation of short antimicrobial peptides with simple amino acid composition Regulatory Peptides. ,vol. 166, pp. 36- 41 ,(2011) , 10.1016/J.REGPEP.2010.08.010
Luyang Cao, Zhongjie Li, Ruhong Zhang, Yingliang Wu, Wenxin Li, Zhijian Cao, StCT2, a new antibacterial peptide characterized from the venom of the scorpion Scorpiops tibetanus. Peptides. ,vol. 36, pp. 213- 220 ,(2012) , 10.1016/J.PEPTIDES.2012.04.010
Shin Saeng Lim, Yangmee Kim, Yoonkyung Park, Jae Il Kim, Il-Seon Park, Kyung-Soo Hahm, Song Yub Shin, The role of the central l- or d-Pro residue on structure and mode of action of a cell-selective α-helical IsCT-derived antimicrobial peptide Biochemical and Biophysical Research Communications. ,vol. 334, pp. 1329- 1335 ,(2005) , 10.1016/J.BBRC.2005.07.029