Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes

摘要: Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction technique with spinning-disk (SD) confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD microscopy, such as fast speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along three dimensions. Both axial lateral can improved simultaneously by virtue blinking/fluctuating nature modified fluorescent probes, exemplified quantum dots. Further, images dual modality obtained through optical fluctuation (SOFI) bleaching/blinking-assisted (BaLM). Therefore, SD-SOFI capturing 100 frames while SD-BaLM yields high-resolution imaging.