IL-8 enhancement of amyloid-beta (Aβ1-42)-induced expression and production of pro-inflammatory cytokines and COX-2 in cultured human microglia
作者： Sonia Franciosi , Hyun B. Choi , Seung U. Kim , James G. McLarnon
DOI: 10.1016/J.JNEUROIM.2004.10.006
关键词: Immunocytochemistry 、 Interleukin 8 、 Microglia 、 Stimulation 、 Chemistry 、 Endocrinology 、 Amyloid beta 、 Cytokine 、 Chemokine 、 Proinflammatory cytokine 、 Internal medicine
摘要: Abstract The effects of the chemokine IL-8 on amyloid beta peptide (Aβ1-42)-induced responses in cultured human microglia were investigated using RT–PCR, ELISA and immunocytochemistry. Aβ1-42 (5 μM) applied for 8 h induced expression increased production pro-inflammatory cytokines IL-6, IL-1β, TNF-α, inducible enzyme COX-2 IL-8. Microglial treatment with added (at 100 ng/mL) led to enhancement both all these factors compared alone. Stimulation itself was effective increasing microglial cytokines, COX-2, however, had no effect protein levels factors. anti-inflammatory IL-10 TGFβ1 remained unchanged from basal stimulation either Aβ1-42, or together actions potentiate Aβ1-42-induced inflammatory mediators may have particular relevance Alzheimer disease brain which exhibits elevated chemokine.
elsevier.com
sci-hub.se 参考文章(42)
Robert Veerhuis, Mariëlle J. Van Breemen, Jeroen J. Hoozemans, Michela Morbin, Jamal Ouladhadj, Fabrizio Tagliavini, Piet Eikelenboom, Amyloid β plaque-associated proteins C1q and SAP enhance the Aβ1-42 peptide-induced cytokine secretion by adult human microglia in vitro Acta Neuropathologica. ,vol. 105, pp. 135- 144 ,(2003) , 10.1007/S00401-002-0624-7
M Corpuz, Brain glia release factors with opposing actions upon neuronal survival. The Journal of Neuroscience. ,vol. 13, pp. 29- 37 ,(1993) , 10.1523/JNEUROSCI.13-01-00029.1993
Hyan B. Choi, C. Khoo, Jae K. Ryu, Edo Van Breemen, Seung U. Kim, James G. McLarnon, Inhibition of lipopolysaccharide‐induced cyclooxygenase‐2, tumor necrosis factor‐α and [Ca2+]i responses in human microglia by the peripheral benzodiazepine receptor ligand PK11195 Journal of Neurochemistry. ,vol. 83, pp. 546- 555 ,(2002) , 10.1046/J.1471-4159.2002.01122.X
T C Saido, S A Frautschy, G M Cole, F Yang, M Irrizarry, B Hyman, K Hsiao, Microglial response to amyloid plaques in APPsw transgenic mice. American Journal of Pathology. ,vol. 152, pp. 307- 317 ,(1998)
D. Mcvicar, J. J. Oppenheim, R. Badolato, Ji Ming Wang, J. A. Johnston, D. J. Kelvin, Luo Ling Xu, Serum amyloid A induces calcium mobilization and chemotaxis of human monocytes by activating a pertussis toxin-sensitive signaling pathway. Journal of Immunology. ,vol. 155, pp. 4004- 4010 ,(1995)
Michael T. Heneka, Vitaliy Gavrilyuk, Gary E. Landreth, M. Kerry O'Banion, Guy Weinberg, Douglas L. Feinstein, Noradrenergic depletion increases inflammatory responses in brain: effects on IκB and HSP70 expression Journal of Neurochemistry. ,vol. 85, pp. 387- 398 ,(2003) , 10.1046/J.1471-4159.2003.01694.X
C. F. Brosnan, J. W. Berman, D. W. Dickson, Wei Liu, S. C. Lee, Cytokine production by human fetal microglia and astrocytes : differential induction by lipopolysaccharide and IL-1β Journal of Immunology. ,vol. 150, pp. 2659- 2667 ,(1993)
Charles Mackay, Megan McNamara, Shixin Qin, Bradley T. Hyman, Mengqi Xia, Interleukin-8 receptor B immunoreactivity in brain and neuritic plaques of Alzheimer's disease American Journal of Pathology. ,vol. 150, pp. 1267- 1274 ,(1997)
John B. Davis, Heather F. McMurray, David Schubert, The amyloid beta-protein of Alzheimer's disease is chemotactic for mononuclear phagocytes. Biochemical and Biophysical Research Communications. ,vol. 189, pp. 1096- 1100 ,(1992) , 10.1016/0006-291X(92)92317-Q
D Walker, Gene expression profiling of amyloid beta peptide-stimulated human post-mortem brain microglia Neurobiology of Aging. ,vol. 22, pp. 957- 966 ,(2001) , 10.1016/S0197-4580(01)00306-2