SUMOylation of PHYTOCHROME INTERACTING FACTOR 3 promotes photomorphogenesis in Arabidopsis thaliana.

摘要: In Arabidopsis thaliana, phytochrome B (phyB) is the dominant receptor of photomorphogenic development under red light. Phytochrome interacts with a set downstream regulatory proteins, including PHYTOCHROME INTERACTING FACTOR 3 (PIF3). The interaction between PIF3 and photoactivated phyB leads to rapid phosphorylation degradation also phyB, events which are required for proper photomorphogenesis. Here we report that SUMOylated at Lys13 (K13) residue could detect this posttranslational modification in heterologous experimental system planta. We found SUMO acceptor site mutant PIF3(K13R) binds more strongly target promoters than its SUMOylated, wild-type counterpart. Seedlings expressing show an elongated hypocotyl response, elevated photoprotection higher transcriptional induction red-light responsive genes compared plantlets PIF3. These observations supported by lower level plants possess only PIF3(K13R), indicating SUMOylation alters photomorphogenesis via regulation levels. conclusion, whereas generally connected different stress responses, it fine-tunes light signalling reducing biological activity PIF3, thus promoting