Differential action of benomyl and methyl-2-benzimidazolecarbamate (MBC) in Saccharomyces pastorianus

摘要: Abstract Benomyl and its breakdown product methyl-2-benzimidazolecarbamate (MBC) are equally toxic to many fungi but differ in toxicity Saccharomyces pastorianus . The two fungicides appear act by different mechanisms this organism, at least short-term experiments. Concentrations of benomyl (1–10 μg/ml) which inhibited growth also glucose or acetate oxidation 50–75% rapidly DNA, RNA protein syntheses. No morphological change occurred the cells after addition toxicant. MBC (10 μg/ml), on other hand, did not inhibit oxidation. syntheses were initially, DNA synthesis was sharply curtailed 1 hr. continued for several hours with only moderate inhibition. MBC-treated became greatly enlarged, somewhat distorted slowly reproduced separate. There a marked loss viability. In long-term experiments delayed (colony formation) longer than MBC, contrast it cause these tests better fungistat poorer fungicide. data suggest that S. acts primary phase metabolism while inhibits cytokinesis mitosis.