Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations.
作者： Natascha Zinovieva , Caroline Lassnig , Dieter Schams , Urban Besenfelder , Eckhard Wolf
关键词: Mammary gland 、 Andrology 、 Lactation 、 Biology 、 Transgene 、 Offspring 、 Expression cassette 、 Recombinant DNA 、 Lagomorpha 、 Endocrinology 、 Insertional mutagenesis 、 Internal medicine
摘要: One transgenic rabbit line was generated carrying a fusion gene consisting of the cDNA for human IGF-1 fused to mammary gland specific expression cassette derived from bovine alpha-S1-casein sequences. Transgene shown be strictly tissue and lactation period specific. The bred six generations. All animals showed stable production biologically active over generations no apparent effect on physiological or reproductive performance observed. absence adverse effects homozygous rabbits suggested insertional mutagenesis. Eight hemizygous offspring analysed produced average 363 ± 12μg/ml (ranging 223 61 484 39 μg/ml) mature in their milk, whereas three 543 41 μg/ml 360 15 678 80 μg/ml). Homozygous huIGF-1 females clearly significantly increased recombinant protein.
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