Astragaloside IV facilitates glucose transport in C2C12 myotubes through the IRS1/AKT pathway and suppresses the palmitate-induced activation of the IKK/IκBα pathway

摘要: Astragaloside IV is a monomer isolated from Astragalus membranaceus (Fisch.) Bunge, which one of the most widely used plant-derived drugs in traditional Chinese medicine for diabetes therapy. In present study, we aimed to examine effects astragaloside IV on glucose C2C12 myotubes and underlying molecular mechanisms responsible these effects. Four-day differentiated were exposed palmitate 16 h order establish model insulin resistance 3H glucose uptake, using 2-Deoxy‑D‑[1,2-3H(N)]-glucose (radiolabeled 2-DG), was detected. added 2 h prior exposure. The translocation transporter 4 (GLUT4) evaluated by subcellular fractionation, expression signaling molecules such as receptor β (IRβ), receptor substrate (IRS)1/protein kinase B (AKT) inhibitory κB kinase (IKK)/inhibitor-κBα (IκBα), are associated with signal transduction, assessed basal or insulin‑stimulated state western blot analysis RT-PCR. We also examined mRNA monocyte chemotactic protein 1 (MCP-1), interleukin 6 (IL-6), tumor necrosis factor α (TNFα) Toll‑like receptor 4 (TLR4). Taken together, findings demonstrated that facilitates transport through mechanism involving IRS1/AKT pathway, suppresses palmitate-induced activation IKK/IκBα pathway.