Therapeutic potential of pravastatin for random skin flaps necrosis: involvement of promoting angiogenesis and inhibiting apoptosis and oxidative stress.

摘要: Background: Random skin flap is frequently used in plastic and reconstructive surgery, but its distal part often occurs ischemia necrosis. Pravastatin (Prava) with bioactivities of pro-angiogenesis, anti-apoptosis anti-oxidative stress, may be beneficial for survival. Materials methods: A modified McFarlane model was performed Sprague-Dawley rats. The animals were divided into the Control Prava groups treated as follows: group injected intraperitoneally 2 mg/kg consecutive 7 days, received an equal volume vehicle daily. On day 7, necrosis flaps observed, while visualization blood flow below tissue surface by Laser Doppler imaging (LDBFI). Then euthanized, levels angiogenesis, apoptosis oxidative stress analyzed. Results: decreased edema compared group, more under LDBFI. treatment increased mean vessels density, elevated expression angiogenic proteins (matrix metallopeptidase 9, vascular endothelial growth factor, Cadherin5) antioxidant (superoxide dismutase 1 (SOD1), nitric oxide synthase, heme oxygenase), apoptotic factors (BAX, CYC, Caspase3). In addition, malondialdehyde content reduced, glutathione level SOD activity after Prava. Conclusion: promotes survival random through induction inhibition stress.