Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction
作者： Y H Datta , P T Adams , W R Drobyski , S P Ethier , V H Terry
DOI: 10.1200/JCO.1994.12.3.475
关键词: Breast cancer 、 Bone marrow 、 Keratin 、 Medicine 、 Pathology 、 Primer (molecular biology) 、 Mammary gland 、 Polymerase chain reaction 、 Chemotherapy 、 Reverse transcriptase
摘要: PURPOSEDetection of occult carcinoma in patients with breast cancer may aid the establishment prognosis and development new therapeutic approaches. To improve on existing methods detection, we have developed a reverse-transcriptase polymerase chain reaction (RT-PCR) assay for keratin 19 (K19) transcripts to identify mammary cells peripheral blood bone marrow cancer.PATIENTS AND METHODSPeripheral-blood or samples obtained from 34 stages I IV 39 control subjects without were screened K19 mRNA by nested primer PCR.RESULTSIn reconstitution experiments, RT-PCR reliably detected 10 1 million normal peripheral-blood mononuclear (PBMN) cells. Four stage had detectable transcript blood. Five six histologically negative biopsies following preablative chemotherapy before autologous ...
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