A neurotoxic prion protein fragment enhances proliferation of microglia but not astrocytes in culture.
作者： David R. Brown , Bernhard Schmidt , Hans A. Kretzschmar
DOI: 10.1002/(SICI)1098-1136(199609)18:1<59::AID-GLIA6>3.0.CO;2-Z
关键词: Central nervous system 、 Scrapie 、 Gliosis 、 Biology 、 Neuroglia 、 Gene isoform 、 Cell biology 、 Microglia 、 Immunology 、 Neurodegeneration 、 Astrocyte
摘要: The scrapie isoform of the prion protein (PrP Sc ) induces pathological changes in central nervous system including neurodegeneration and gliosis. A synthetic (PrP) peptide corresponding to amino acid residues 106-126 has been shown be toxic neurons that express PrPC, cellular PrP. Here we show mixed glial cultures PrP106-126 astroglial proliferation is dependent on PrP c expression. In purified subtypes only microglia proliferated response PrP106-126. This effect was independent Destruction by L-leucine methyl ester (LLME) treatment abolished enhanced caused proliferative can restored co-culturing LLME-treated astrocytes with microglia. Microglia therefore seem mediate exerted astrocytes.
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