Chronic hypoxia inhibits tetrahydrobiopterin-induced NO production in rat lungs.
作者： Karel Koubský , Jana Ďurišová , Dana Miková , Jan Herget
DOI: 10.1016/J.RESP.2012.11.012
关键词: Oxidative stress 、 Superoxide 、 Hypoxia (medical) 、 Endocrinology 、 Hypoxic pulmonary vasoconstriction 、 Biology 、 Pulmonary hypertension 、 Internal medicine 、 In vivo 、 Nitric oxide 、 Tetrahydrobiopterin 、 Anesthesia
摘要: Abstract Tetrahydrobiopterin (BH4) is an essential cofactor for nitric oxide synthases (NOS). Oxidative stress oxidises BH4 to dihydrobioptein (BH2), resulting in the uncoupling of two enzymatic domains NOS and production superoxide rather than NO (NOS uncoupling). known be increased early stage chronic hypoxia. This study investigated participation phase hypoxia-induced pulmonary hypertension rats. Rats were exposed 10% O 2 4 days. We effect vitro on isolated rat lungs peripheral blood vessels vivo exhaled concentration air. attenuated hypoxic vasoconstriction its was reversed by l -NAME inhibitor). The main finding that smaller rats days hypoxia normoxic controls. similar vessels. both increase blunted -NIL (specific iNOS inhibitor) therefore attributable iNOS. conclude was, however, assume due decreased BH4/BH2 ratio from this condition.
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