pH sensing in skin tumors: Methods to study the involvement of GPCRs, acid-sensing ion channels and transient receptor potential vanilloid channels.

摘要: Solid tumors exhibit an inversed pH gradient with increased intracellular (pHi ) and decreased extracellular (pHe ). This inside-out is generated via sodium/hydrogen antiporter 1, vacuolar-type H + ATPases, monocarboxylate transporters, (bi)carbonate (co)transporters carboanhydrases. Our knowledge on how pHe -signals are sensed what the respective receptors induce inside cells scarce. Some pH-sensitive (GPR4, GPR65/TDAG8, GPR68/OGR1, GPR132/G2A, possibly GPR31 GPR151) ion channels (acid-sensing ASICs, transient receptor potential vanilloid TRPVs) transduce signals cells. As little known expression function of these sensors, we used immunostainings to study tissue samples from common rare skin cancers. current future work directed towards investigating impact all pH-sensing in different using cell culture techniques selective knockdown/knockout (siRNA/CRISPR-Cas9). To migration proliferation, novel impedance-based wound healing assays have been developed used. The field sensing wounds holds great promise for development pH-targeting therapies, either against regulators or sensors inhibit proliferation migration.