Antimicrobial Activity of Bacteriocins Isolated from Lactic Acid Bacteria Against Resistant Pathogenic Strains

摘要: This study was carried out to treat the microbial diseases in natural way and reduced antibiotic resistance pressure. Five species of Lactic acid bacteria namely, L. acidophilus, bulgaricus, dulbrueckii plantarum, fermentum, were isolated from locally produced yogurt. Bacteriocins MRS broth culture theses through ammonium sulphate precipitation method. Extracted bacteriocins tested against test strain i.e E.coli ATCC 25922 obtain optimum pH, storage temperature heating for maximum antibacterial activity. For this (2-12) pH range, temperatures (-20 °C, 4 °C 37 1 month) (60 100 121 15 minutes) tested. Later activity these at concluded conditions each other as well different pathogens viz. Methicillin-Resistant-Staphylococcus-aureus, E.coli, Salmonella Staphylococcus aureus by using diffusion agar It found that activities 6 temperature. Maximum after 60 compared °C. lactobacilli harmless any heat temperatures. Antibacterial extracted L.acidophilus showed pathogenic compare others. The present has highlighted role indicating their potential a variety human animal diseases.