Adaptive Immunity Restricts Replication of Novel Murine Astroviruses

摘要: The mechanisms of astrovirus pathogenesis are largely unknown, in part due to a lack small-animal model disease. Using shotgun sequencing and custom analysis pipeline, we identified two novel astroviruses capable infecting research mice, murine (MuAstV) STL1 STL2. Subsequent revealed the presence at least additional viruses (MuAstV STL3 STL4), suggestive diverse population mice. Complete genomic characterization subsequent phylogenetic showed that MuAstV STL4 members mamastrovirus genus likely new genogroup. Rag1−/− mice deficient B T cells, demonstrate adaptive immunity is required control infection. Furthermore, using Stat1−/− innate signaling, role for immune response replication. Our results STL permits study infection host-pathogen interactions genetically manipulable model. Finally, detected commercially available suggesting these may be present academic commercial mouse facilities, with possible implications interpretation data generated current models