作者: Harold H. Schmitz , Christopher L. Poor , Eric T. Gugger , John W. Erdman
DOI: 10.1016/0076-6879(93)14058-Q
关键词: Quantitative analysis (chemistry) 、 Carotenoid 、 Chromatography 、 Biology 、 Separation method
摘要: Publisher Summary This chapter describes the extraction of carotenoids from human and animal tissues subsequent analysis using high-performance liquid chromatography (HPLC). The identification quantitation are routinely accomplished for many types plant HPLC techniques, careful examination these methods is helpful in developing approaches to analyze carotenoids. An accurate qualitative quantitative present dependent on selection analytical procedures. Strict adherence laboratory protocol, which minimizes degradation and/or isomerization during both procedures, should be observed ensure results. procedures modifications presented provide a method by major may accurately identified quantified.