Method of determining base sequence of DNA or RNA using heavy element labeling and imaging by transmission electron microscopy
作者： Kuniaki Nagayama
DOI:
关键词: DNA 、 Transmission electron microscopy 、 DNA sequencing 、 Single molecule real time sequencing 、 Molecular biology 、 A-DNA 、 Biological system 、 RNA 、 Biology 、 Electrophoresis 、 DNA sequencer
摘要: Novel DNA sequence determination method and sequencer system providing a sequencing speed 10 3 to 4 times faster than the current (10 5 bases per day with lane at maximum) of existing based on electrophoresis. The includes step discriminating base-specific labels heavy elements using magnified image elongated single-chain or RNA produced by transmission electron microscope (TEM). uses this method. invention provides that is higher orders magnitude.
lens.org 参考文章(8)
Kuniaki Nagayama, Radostin Danev, Thin-film phase plate, phase-contrast electron microscope using same, and method of preventing charging of phase plate ,(2001)
Yoshinori Harada, Yuji Sasaki, Hydrogen bond labeling and base sequence determination methods for DNA or RNA ,(1993)
David Klenerman, Shankar Balasubramanian, Mark Allen Osborne, Colin Barnes, Arrayed biomolecules and their use in sequencing European Patent Office. ,(1999)
Radoje Drmanac, Methods and compositions for detection or quantification of nucleic acid species ,(1998)
E. N. Moudrianakis, M. Beer, BASE SEQUENCE DETERMINATION IN NUCLEIC ACIDS WITH THE ELECTRON MICROSCOPE, III. CHEMISTRY AND MICROSCOPY OF GUANINE-LABELED DNA Proceedings of the National Academy of Sciences of the United States of America. ,vol. 53, pp. 564- 571 ,(1965) , 10.1073/PNAS.53.3.564
Hiroyuki TANAKA, Tomoji KAWAI, Visualization of DNA by Scanning Probe Microscopy. Seibutsu Butsuri. ,vol. 40, pp. 336- 340 ,(2000) , 10.2142/BIOPHYS.40.336
Stephen P. A. Fodor, William J. Dower, Dennis W. Solas, Method of detecting nucleic acids ,(1996)
Nagayama Kuniaki, COMPLEX SIGNAL DETECTING METHOD, COMPLEX MICROSCOPE AND COMPLEX DIFFRACTION DEVICE ,(1999)