Characterization of Fasciola samples from different host species and geographical localities in Spain by sequences of internal transcribed spacers of rDNA
作者： S. Alasaad , C. Q. Huang , Q. Y. Li , J. E. Granados , C. García-Romero
DOI: 10.1007/S00436-007-0628-2
关键词: Genetic marker 、 Fasciola gigantica 、 Fasciola 、 Biology 、 Population 、 Ribosomal DNA 、 Fasciola hepatica 、 Genetics 、 Liver fluke 、 Hepatica
摘要: In the present study, 25 samples representing Fasciola (Platyhelminthes: Trematoda: Digenea) from nine host species and 19 geographical locations in Spain were characterized genetically by sequences of first (ITS-1) second (ITS-2) internal transcribed spacers (ITS) nuclear ribosomal DNA (rDNA). The ITS rDNA was amplified individual liver flukes polymerase chain reaction (PCR), amplicons sequenced directly. lengths ITS-1 ITS-2 422 362 bp, respectively, for all Spanish fluke sequenced. Comparison examined study with that hepatica, gigantica "intermediate Fasciola" revealed represent single F. only slight sequence variation (1/362, 0.3%) among samples, but not related to particular and/or origins samples. hepatica differed elsewhere two nucleotides ITS-2, which provided genetic marker differentiation other localities. These results have implications studying population structure diagnosis control disease it causes.
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