Fusarium verticillioides (Saccardo) Nirenberg associated with hardlock of cotton.

摘要: Boll rots of cotton (Gossypium hirsutum L.) are common in the humid areas Southeastern US. One type boll damage that may be differentiated from others is hardlock, with symptoms include compression fibers within individual locules mature, open bolls without further obvious disintegration lint or to carpel wall. The principal economic effect boll’s unharvestable by mechanical pickers. This disease endemic Southeast and can cause severe yield losses up 70% some fields. Scanning electron microscopy images hardlocked showed flattened twisted tissue compared healthy bolls. Fusarium verticillioides (Saccardo) Nirenberg was fungus most commonly isolated seeds developing Flowers inoculated F. on day bloom spraying a spore suspension onto flowers developed significantly (P < 0.05) more hardlock untreated controls. infection process analyzed using isolate tagged green fluorescent protein (GFP). When it applied bloom, GFP-tagged strain detected stigma style 2 days after (DAB) at 4, 6, 8, 10, 16, 20, 40, 60 (open bolls) DAB. By 8 DAB, GFP over 80% seeds.