Regulation of ecdysteroid biosynthesis in crayfish Y-organs: I. Role of cyclic nucleotides
作者： Dietrich Sedlmeier , Renate Fenrich
关键词: Biology 、 Endocrinology 、 IBMX 、 Orconectes limosus 、 Second messenger system 、 Internal medicine 、 Endocrine gland 、 Nucleotide 、 Ecdysteroid 、 Cyclic nucleotide 、 Forskolin 、 Animal Science and Zoology 、 General Medicine
摘要: Ecdysteroidogenesis in crustacean Y-organs is negatively regulated by the so-called molt-inhibiting hormone (MIH), which thought to inhibit ecdysteroid pathway either directly through phosphorylation of key enzyme(s) and/or action on synthesis these enzymes. In present study role cyclic nucleotides mode MIH astacuran Orconectes limosus was investigated. were incubated vitro with sinus gland conditioned medium (SGCM) and AMP (cAMP) GMP (cGMP) levels measured. A striking increase cGMP values (4 43 pmoles/mg protein) observed after 2 hours, whereas cAMP unchanged. Using established bioassay for measurement inhibitory release from (Soumoff O'Connor, '82) potential dBcAMP, dBcGMP, forskolin, IBMX diminish this demonstrated, suggesting a possible both MIH. The findings are discussed view effect MIH-material nucleotide crab (Mattson, '86). © 1993 Wiley-Liss, Inc.
sci-hub.se 参考文章(29)
Mark P. Mattson, New insights into neuroendocrine regulation of the crustacean molt cycle Zoological Science. ,vol. 3, pp. 733- 744 ,(1986)
J.F. Kuo, G.R. Wyatt, Paul Greengard, Cyclic Nucleotide-dependent Protein Kinases IX. PARTIAL PURIFICATION AND SOME PROPERTIES OF GUANOSINE 3',5'-MONOPHOSPHATE-DEPENDENT AND ADENOSINE 3',5'-MONOPHOSPHATE-DEPENDENT PROTEIN KINASES FROM VARIOUS TISSUES AND SPECIES OF ARTHROPODA Journal of Biological Chemistry. ,vol. 246, pp. 7159- 7167 ,(1971) , 10.1016/S0021-9258(19)45868-7
Dietrich Sedlmeier, Rainer Keller, The mode of action of the crustacean neurosecretory hyperglycemic hormone General and Comparative Endocrinology. ,vol. 45, pp. 82- 90 ,(1981) , 10.1016/0016-6480(81)90172-6
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
David B. Morton, James W. Truman, Steroid regulation of the peptide-mediated increase in cyclic GMP in the nervous system of the hawkmoth, Manduca sexta. Journal of Comparative Physiology A-neuroethology Sensory Neural and Behavioral Physiology. ,vol. 157, pp. 423- 432 ,(1985) , 10.1007/BF00615142
Mark P. Mattson, Eugene Spaziani, Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production Molecular and Cellular Endocrinology. ,vol. 42, pp. 185- 189 ,(1985) , 10.1016/0303-7207(85)90106-6
John F. McCarthy, Ecdysone metabolism in premolt land crabs (Gecarcinus lateralis) General and Comparative Endocrinology. ,vol. 47, pp. 323- 332 ,(1982) , 10.1016/0016-6480(82)90239-8
Rainer Keller, Axel Willig, Experimental evidence of the molt controlling function of the Y-organ of a macruran decapod,Orconectes limosus Journal of Comparative Physiology B-biochemical Systemic and Environmental Physiology. ,vol. 108, pp. 271- 278 ,(1976) , 10.1007/BF00691675
Susumu Y. Takahashi, Kazunori Hanaoka, Multiple protein kinases in the American cockroach, Periplaneta americana Insect Biochemistry. ,vol. 7, pp. 133- 139 ,(1977) , 10.1016/0020-1790(77)90006-3
Eugene Spaziani, Huw H. Rees, Wenan L. Wang, R.Douglas Watson, Evidence that Y-organs of the crab Cancer antennarius secrete 3-dehydroecdysone. Molecular and Cellular Endocrinology. ,vol. 66, pp. 17- 25 ,(1989) , 10.1016/0303-7207(89)90044-0