Chapter 23. Affinity Labeling of Hormone Binding Sites

摘要: Publisher Summary This chapter discusses affinity labeling (AL) of hormone binding sites. AL is a technique for covalently protein In this method, reactive functional group incorporated within the structure ligand molecule in such manner that modified bound by and reacts with amino acid residues or near site, forming covalent bond through attaching function. The salient feature an process formation takes place complex reagent high, localized concentration results enhanced reaction between nearby residues, which basis selectivity. reagent, particularly if non-polar, may associate low-affinity, non-specific hydrophobic regions other proteins, can take complex. 16α- 11α-Bromoacetoxyprogesterone 20-acryloxyprogesterone irreversibly inactivate 11β- 21-hydroxylase activities rat adrenal progesterone protect against effect 16α 20-derivatives.