CNS projections to the pterygopalatine parasympathetic preganglionic neurons in the rat : a retrograde transneuronal viral cell body labeling study

摘要: Abstract The retrograde transneuronal viral cell body labeling method was used to study the CNS nuclei that innervate parasympathetic preganglionic neurons which project pterygopalatine ganglion. Small injections of a suspension pseudorabies virus (PRV) were made in ganglion rats and after 4 days their brains processed for immunohistochemical detection PRV. Some tissues stained with dual immunofluorescence permitted visualization PRV neurotransmitter enzyme or serotonin immunoreactivity same cell. Retrograde detected ipsilateral ventrolateral medulla oblongata region has been termed superior salivatory nucleus. This area retrogradely labeled Fluoro-Gold dye transneuronally infected bodies provide putative afferent inputs pytergopalatine mapped throughout brain. In oblongata, seen nucleus tractus solitarii, dorsomedial part spinal trigeminal gigantocellular reticular most experiments, some A1 catecholamine cells raphe magnus, pallidus, obscurus, parapyramidal labeled. pons, found parabrachial nucleus, A5 group, non-catecholamine subcoeruleus region. midbrain, located central gray matter retrorubral field. diencephalon, mainly hypothalamus. areas included lateral hypothalamic area, preoptic paraventricular nuclei, ventral zona incerta. Contralateral second order tuberomammillary these histidine decar☐ylase-immunoreactive. forebrain, bed stria terminalis, substantia innominata, an cerebral cortex called amygdalopiriform transition zone