An insertion within a variably spliced Drosophila tropomyosin gene blocks accumulation of only one encoded isoform
作者： Christine C. Karlik , Eric A. Fyrberg
DOI: 10.1016/0092-8674(85)90061-3
关键词: Intron 、 Biology 、 RNA splicing 、 Genetics 、 Gene 、 Gene isoform 、 Tropomyosin 、 Exon 、 Allele 、 Mutation 、 General Biochemistry, Genetics and Molecular Biology
摘要: We have characterized an aberrant allele of a variably spliced Drosophila tropomyosin gene. The was recovered from the flightless Ifm(3)3 strain, which has been shown to structurally and functionally abnormal indirect flight muscles. transcribed portion mutant gene is interrupted by 8,8 kb insertion middle repetitive DNA having structure typical copia-like mobile elements. positioned so as interrupt exon sequence in one splicing mode and, simultaneously, intron alternate mode. As consequence fails direct synthesis muscle-specific isoform, but remains capable specifying second accumulates nonfibrillar
elsevier.com
sci-hub.se 参考文章(33)
M Periasamy, E E Strehler, L I Garfinkel, R M Gubits, N Ruiz-Opazo, B Nadal-Ginard, Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing. Journal of Biological Chemistry. ,vol. 259, pp. 13595- 13604 ,(1984) , 10.1016/S0021-9258(18)90735-0
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
Kaname MOGAMI, Shinobu C. FUJITA, Yoshiki HOTTA, Identification of Drosophila Indirect Flight Muscle Myofibrillar Proteins by Means of Two-Dimensional Electrophoresis Journal of Biochemistry. ,vol. 91, pp. 643- 650 ,(1982) , 10.1093/OXFORDJOURNALS.JBCHEM.A133736
W. Bender, M. Akam, F. Karch, P. A. Beachy, M. Peifer, P. Spierer, E. B. Lewis, D. S. Hogness, Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. ,vol. 221, pp. 23- 29 ,(1983) , 10.1126/SCIENCE.221.4605.23
Russell M. Medford, Hanh T. Nguyen, Antonia T. Destree, Eric Summers, Bernardo Nadal-Ginard, A novel mechanism of alternative RNA splicing for the developmentally regulated generation of troponin T isoforms from a single gene Cell. ,vol. 38, pp. 409- 421 ,(1984) , 10.1016/0092-8674(84)90496-3
M. P. Snyder, D. Kimbrell, M. Hunkapiller, R. Hill, J. Fristrom, N. Davidson, A transposable element that splits the promoter region inactivates a Drosophila cuticle protein gene Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 7430- 7434 ,(1982) , 10.1073/PNAS.79.23.7430
Robert Levis, Kevin O'Hare, Gerald M. Rubin, Effects of transposable element insertions on RNA encoded by the white gene of Drosophila Cell. ,vol. 38, pp. 471- 481 ,(1984) , 10.1016/0092-8674(84)90502-6
Allan C. Spradling, Anthony P. Mahowald, Identification and genetic localization of mRNAs from ovarian follicle cells of Drosophila melanogaster Cell. ,vol. 16, pp. 589- 598 ,(1979) , 10.1016/0092-8674(79)90032-1
Yo-ichi Nabeshima, Yoshiaki Fujii-Kuriyama, Masami Muramatsu, Kikuo Ogata, Alternative transcription and two modes of splicing result in two myosin light chains from one gene Nature. ,vol. 308, pp. 333- 337 ,(1984) , 10.1038/308333A0